Neurolucida Tracing

[Meena]

Hi,

I am working on putting together a small document on good tracing techniques and considerations when tracing a cell using Neurolucida.

From my reading so far, there is a lot of emphasis on good microscope setup and calibration. Also, there is much stress of the need for shrinkage correction to obtain accurate data.

From my reading, there is also a lot of literature out there ( De Shutter etc ) that clearly shows of small variations in parameters like the diameter of a dendrite in the anatomical data that can significantly alter our estimations of Cm, Rm Ra when modelling.

I would like to know what the comments and suggestions the Neuron Forum has on good tracing techniques and standards esp when using tools like Neurolucida to trace Neurons. I know there are many out there who have better tracing skills and perhaps your skills can help those of us who have less experience in tracing neurons.

Thanks,
Meena