My question is somehow related to the Pascal's question viewtopic.php?f=16&t=4084 because, it seems, I stumbled to a similar problems.

I'm trying to play around with Iavarone's model, which uses the same old Destexhe's code https://senselab.med.yale.edu/ModelDB/S ... 881#tabs-1. At least in two modules for low and high threshold calcium currents read inner and outer calcium concentrations (

**cai**,

**cao**) and compute the GHK equation. These two global segment's variables are listed in the parameters block:

Code: Select all

```
:from TC_iL.mod
PARAMETER {
v (mV)
celsius (degC)
dt (ms)
cai = 0.5E-4 (mM) : Value from Amarillo et al., J Neurophysiol, 2014
cao = 2 (mM)
pcabar= 1e-4 (cm/s)
}
:from TC_ITGHK_Des98.mod
PARAMETER {
v (mV)
:celsius = 36 (degC)
celsius (degC) : EI
pcabar =.2e-3 (cm/s) : Maximum Permeability
shift = 2 (mV) : corresponds to 2mM ext Ca++
actshift = 0 (mV) : shift of activation curve (towards hyperpol)
cai = 2.4e-4 (mM) : adjusted for eca=120 mV
cao = 2 (mM)
qm = 2.5 : Amarillo et al., J Neurophysiol, 2014
qh = 2.5 : Amarillo et al., J Neurophysiol, 2014
}
```

However, it seems, NEURON ignores these values:

Code: Select all

```
nocmodl" TC_ITGHK_Des98
Translating TC_ITGHK_Des98.mod into TC_ITGHK_Des98.c
Warning: Default 2 of PARAMETER cao will be ignored and set by NEURON.
Warning: Default 0.00024 of PARAMETER cai will be ignored and set by NEURON.
Thread Safe
```

**cai**and

**cao**should be in

*ASSIGNED*block without any values attached and all default settings should be done in a compartment? Something like this in hoc

Code: Select all

```
soma cai = 2.4e-4 // default variable adjusted for eca=120 mV
soma cao = 2
```

Code: Select all

```
soma(0.5).cai = 2.4e-4 # default variable adjusted for eca=120 mV
soma(0.5).cao = 2
```