Is it possible to plot the total net current during action potential?
I could plot current of Na or K but I need the whole current.
Bests
Loffler Yourganov
Total current during action potential
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Re: Total current during action potential
Yes, but you must define what you mean by "total net current." Do you mean "total membrane current" or sum of membrane ionic currents?Lofflery wrote:Is it possible to plot the total net current during action potential?
I could plot current of Na or K but I need the whole current.
If you mean "total membrane current" (sum of ionic and capacitive current) the easiest thing to do is to insert the extracellular mechanism. Then you can plot i_membrane. This is easy, but there is one caveat: i_membrane does not include contributions from ELECTRODE_CURRENT point processes. Examples of such point processes include IClamps, SEClamps, and VClamps. If in doubt about any point process, examine the NMODL source code
If you mean "sum of membrane ionic currents" you have to add them up yourself. If you are using
NEURON Main Menu / Graph / Current axis
enter the appropriate expression into the "Plot what" tool's edit field. For example, in a section that contains only the hh mechanism, you would type
Code: Select all
ina+ik+il_hh
Code: Select all
g.addexpr("ina+ik+il_hh")
Re: Total current during action potential
Hi Dr. Carnevale,
Thank you so much for your detailed explanation. This helps me a lot, although this post has been inactive for 8 years.
I am trying to achieve the same thing, monitoring the total membrane ionic currents, but with nonspecific current and SEClamp.
I am little bit puzzled because I am not sure if there is any electrode current with SEClamp. Also I am sure how it will affect the nonspecific current.
What would be the best way to keep track of total membrane ionic current in my case?
Thank you again.
Min.
Thank you so much for your detailed explanation. This helps me a lot, although this post has been inactive for 8 years.
I am trying to achieve the same thing, monitoring the total membrane ionic currents, but with nonspecific current and SEClamp.
I am little bit puzzled because I am not sure if there is any electrode current with SEClamp. Also I am sure how it will affect the nonspecific current.
What would be the best way to keep track of total membrane ionic current in my case?
Thank you again.
Min.
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- Posts: 6305
- Joined: Wed May 18, 2005 4:50 pm
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Re: Total current during action potential
The current injected by an SEClamp is an electrode current.mschoi2 wrote:I am little bit puzzled because I am not sure if there is any electrode current with SEClamp.
Why not do the experiment and find out? And, of course, let me know what you discover. Suggest you do this with a simple model--just one or two compartments, max.Also I am sure how it will affect the nonspecific current.