Using NetStim

[Brody M]

Hi,

I've tried using NetStim as an AP generator by connecting it to a biophysical cell through an ExpSyn but it doesn't generate AP's at all.

Attached is my code:

Code: Select all

///Using NetStim////

	load_file("nrngui.hoc")

////topology////

	create soma

////geometry////

	diam= 30
	L = 30
	nseg = 1
	
////biophysics////

	Ra = 100
	cm = 1	

	soma insert hh

////Creating a NetStim ////
	objref netstim
	netstim= new NetStim(0.5)
	netstim.interval = 100 //(ms)
	netstim.number = 10
	netstim.start = 100 //(ms)
	netstim.noise = 0
	
// Attaching the cell to the an ExpSyn synapse//
	objref syn
	soma syn = new ExpSyn (0.5)

// Attaching the NetStim to the synapse//
	objref netcon
	netcon= new NetCon(netstim, syn)
	
////Recording////
	objref g
	g = new Graph()
	g.beginline()
	t=0
	dt=0.025
	for i= 0, 40000{
		g.line(t,soma.v(0.5))
		fadvance()
	}

I can't understand why it's not working, any suggestions?

Thank you very much
Moran

[ted]

I've tried using NetStim as an AP generator by connecting it to a biophysical cell through an ExpSyn but it doesn't generate AP's at all.

How do you know this?