Problems with adding paired pulse stimulations
Posted: Thu Dec 12, 2013 9:35 am
Hi Ted,
I am trying to examine the inactivation recovery rate of a slowly inactivating potassium chanel based on our wet-lab experiment result.
The experiment is conducted using paired-pulse recovery protocol where we stimulates the cell with an depolarizing 20mV for 100ms after holding at -70mV. We repeat several times with a fixed time interval to examine the rate of the ion channel recovery from inactivation.
SEClamp allows only one round of stimulation (with 3 different voltages), and it seems that I couldn't use this for a train of stimulation. I checked in the 'hot tips' and 'frequently asked questions' and I found your suggestions on downloading 'pulsedistrib' for pulses with defined intervals. I have been struggling to use ipulse model from 'pulsedistrib' to model my 'paired pulse recovery experiment'. I am new with NEURON and I am not sure whether I am in the right direction.
Now, my questions are:
1. Are there better ways to model the 'paired pulse stimulation with voltage clamp rather than IClamp?
2. Is the 'pulsedistrib' the right one to refer to?
Cheers!
xianghua.
I am trying to examine the inactivation recovery rate of a slowly inactivating potassium chanel based on our wet-lab experiment result.
The experiment is conducted using paired-pulse recovery protocol where we stimulates the cell with an depolarizing 20mV for 100ms after holding at -70mV. We repeat several times with a fixed time interval to examine the rate of the ion channel recovery from inactivation.
SEClamp allows only one round of stimulation (with 3 different voltages), and it seems that I couldn't use this for a train of stimulation. I checked in the 'hot tips' and 'frequently asked questions' and I found your suggestions on downloading 'pulsedistrib' for pulses with defined intervals. I have been struggling to use ipulse model from 'pulsedistrib' to model my 'paired pulse recovery experiment'. I am new with NEURON and I am not sure whether I am in the right direction.
Now, my questions are:
1. Are there better ways to model the 'paired pulse stimulation with voltage clamp rather than IClamp?
2. Is the 'pulsedistrib' the right one to refer to?
Cheers!
xianghua.