About shrinkage correction
Posted: Thu Apr 09, 2009 6:46 am
Dear Forum,
I am a new user of Neurolucida software for neuron reconstruction and an old user of NEURON software, and I would really apreciate if I could have your opinion on an emerged issue.
I have been through the maping of the neuron, and now I am about to assemble the dendritic segments belonging to different sections.
Since my slides were treated differently, I have to apply shrinkage correction (SC) for some of them. Neurolucida help recommends that "under normal conditions, SC only be used in Neurolucida to adjust for z, unless sections are mounted upside down". Looking at the orthogonal view of the reconstructed neuron I can say that some slides have been shrunk compared to the rest and to the pre-embedded section thickness, as far as this concerns shrinkage at the z axis. However, I only have rough estimation (SC~1.1) for the x, y shrinkage.
Having in mind that I need a precise maping of the dendritic lengths, should I apply SC at x and y as well? Additionaly, If I perform x, y SC nothing matches!
Thank you very much for your time,
Eleftheria
I am a new user of Neurolucida software for neuron reconstruction and an old user of NEURON software, and I would really apreciate if I could have your opinion on an emerged issue.
I have been through the maping of the neuron, and now I am about to assemble the dendritic segments belonging to different sections.
Since my slides were treated differently, I have to apply shrinkage correction (SC) for some of them. Neurolucida help recommends that "under normal conditions, SC only be used in Neurolucida to adjust for z, unless sections are mounted upside down". Looking at the orthogonal view of the reconstructed neuron I can say that some slides have been shrunk compared to the rest and to the pre-embedded section thickness, as far as this concerns shrinkage at the z axis. However, I only have rough estimation (SC~1.1) for the x, y shrinkage.
Having in mind that I need a precise maping of the dendritic lengths, should I apply SC at x and y as well? Additionaly, If I perform x, y SC nothing matches!
Thank you very much for your time,
Eleftheria