Step 2. Load the Experimental Data into the Multiple Run Fitter

Actually, we'll be loading the data into the Multiple Run Fitter's Function Fitness Generator.


A. We need to load our data into the Function Fitness Generator.

The easiest way to load data into a Function Fitness Generator is to read the data into NEURON's clipboard, and then paste it into the Generator.

NEURON Main Menu / Vector / Retrieve from File
   brings up a tool that we can use to navigate the directory tree and choose the file that contains the data.

The data must be plain ASCII, with the following contents :
  1. Optional line with the format
    label:anystring
  2. Optional "count" line with a number that specifies how many x y data pairs follow.
  3. The actual data, consisting of one tab-separated x y data pair per line.
Retrieval of long files is much more efficient if the "count" is present.
If the "count" is absent, there must be no empty lines at the end of the file and the last character must be a newline.
Example :
label:soma.v(.5)
201
0       -70
0.025   -65.8269
 ...etc. for 199 more lines

Double click on gsyn.dat, the file that contains the data.

In the Generator, click on Regions / Data from Clipboard

Our data appear as the red trace.
The vertical blue lines are cursors that can be used to select regions to which different weights will be applied when fitting a function to the red trace. For this example we will leave these alone.

These data were generated by the ampakin mechanism (using the model files
provided in the accompanying synap.zip) and saved to gsyn.dat manually
by Pick Vector followed by Vector / Save to File.


Time to save everything to a session file again.


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Copyright © 2003-2004 by N.T. Carnevale and M.L. Hines, All Rights Reserved.